Research results that 'Can treat diabetes' is released with caffeine of coffee and tea as a trigger
by Zach Inglis
It is said that caffeine contained in coffee, tea, green tea etc. has "the effect of waking up ", while it is said that excessive ingestion adversely affects the body. By administering such caffeine to a diabetic animal incorporating a special synthetic gene, research results that caffeine can be used as a cell trigger and "can treat diabetes" was announced.
Caffeine-inducible gene switching controlling experimental diabetes | Nature Communications
https://www.nature.com/articles/s41467-018-04744-1
Scientists use caffeine to control genes-and treat diabetic mice with coffee | Ars Technica
https://arstechnica.com/science/2018/06/can-coffee-treat-diabetes-it-can-in-mice-with-this-synthetic-genetic-implant/
A research team led by Professor Martin Fussenegger who is conducting research on bioengineering at the Zurich Institute of Technology is a system in which a synthetic gene system is expressed as a result of the administration of specific substances in the body of animals, I am studying. Fussenegger, who thought of avoiding things that could possibly cause side effects on the body such as bacteria and intense antibiotics when selecting substances to be administered into the body, is a familiar substance that can be produced cheaply without toxicity I thought about using "caffeine" as a trigger.
To create genes that detect caffeine in the body of animals, the research team used " caffeine-reactive antibodies (aCaffVHH)" in the camel's body. aCaffVHH has the property that in response to caffeine, two aCaffVHH bind to one ( dimerize ) and embrace caffeine molecule in it. The research team utilizes the characteristic of "a caff VHH" when detecting "caffeine and dimerize", it fuses a protein which functions "by dimerizing" to aCaff VHH, and the animal We conducted experiments to make specific work in the body.
The research team decided to modify the gene so that it produces the enzyme called SEAP when it dimers, controlling the cell proliferation, signal transduction and transcription activating factor 3 (STAT 3 ), and embed it in the antibody. SEAP is capable of cleaving specific substrates that can be sprayed directly onto cells, and the substrate emits light when cleaved by SEAP. In other words, if researchers could embed antibodies fused with genetically altered STAT3 in the cells and see how the cells were lit, it would be "activated by STAT3 dimerizing and produced SEAP" You can see.
The research team embeds an antibody incorporating genetically modified STAT3 in an immortalized stem cell and conducted experiments. Even in slight caffeine contained in beverages such as Starbucks' coffee, Red Bull, Coca-Cola, etc., stem cells respond sensitively It seems to have demonstrated that it emits light.
by winhide
Subsequently, the research team decided to investigate whether "caffeine works" using antibodies incorporating more useful genes. The research team watched a hormone that promotes secretion of insulin called GLP-1 which is also used as a therapeutic agent for diabetes. We modified the gene to produce GLP-1 by dimerization of STAT 3, and fused STAT 3 genetically modified to aCaff VHH into a permeable capsule to develop type 2 diabetes I embedded in the body of a mouse.
Continued administration of coffee made with Nespresso of espresso machine to mice implanted with permeable capsules resulted in caffeine penetrating into the capsules and activating STAT 3 to produce GLP-1 did. Type 2 diabetes mice whose secretion of insulin was promoted by the effect of GLP-1 said that the blood glucose level dropped to almost the same blood glucose level as in normal mice.
Fussenegger said about the results of the study, "Mice given coffee on a continuous basis did not cause problems such as heart rate rise and blood glucose levels falling to dangerous values", triggering caffeine It is said that diabetes treatment is highly safe. It has also been proven that insulin is not secreted in drinks not containing caffeine. There is no doubt that a long step is necessary from the experimental stage of the mouse until it is actually used for human treatment, but Fussenegger says, "By using beverages that people ever daily speak, It will be possible. "
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