Real-time PCR thermal cycler "Open qPCR" which can easily identify DNA

Thermal cyclerWhat'sPolymerase chain reactionOne of the devices to amplify DNA by. Although it is an expensive device with more than 20,000 dollars (about 2.3 million yen) per unit with high accuracy, an inexpensive real-time PCR thermal cycler designed for open source in Kickstarter "Open qPCR"Has appeared.

Chai Biotechnologies
http://www.chaibio.com/

Real time PCR thermal cycler "Open qPCR" details can be seen from the following movie.


The genes of all organisms are said to be "ultimate personal information", for example, by analyzing human genetic information, it becomes possible to understand the appropriateness of potential exercise capacity.


In addition, it is possible to confirm the presence or absence of a pathogen by analyzing the DNA of food.


However, analysis of DNA can not be done easily, and inspection by facilities and experts is necessary.


Therefore, in countries where medical facilities are not sufficient, even though signs of pandemic due to plague can be seen, inspection itself may not be possible.


So Chai Biotechnologies developed a real-time PCR (SYBR Green method) To amplify the DNAThermal cycler"Open qPCR" is. The thermal cycler has various discrimination methods, but the performance of Open qPCR is moderate.


By connecting the LAN · USB port on the back of the main unit to the PC, it is possible to analyze the data of Open qPCR with software. Both hardware and dedicated software are all made open source.


The body is equipped with a touch display, you can operate like a smartphone.


When the amplification is completedOptical systemDNA will be detected in the right test tube. Since DNA other than DNA enters the left test tube, it will not shine in fluorescent color.


Although it can not detect chemical substances, it can detect various microorganisms and viruses besides DNA.


If you are a dog breeder, you can investigate the DNA of the breeding dog to reference for training ......


Ebola virus can be examined quickly. Although this equipment does not support multiplex real-time PCR, it will be added as a new function in the future.


With dedicated software like this, it is possible to diagnose DNA with PC.

ACTN 3
In addition, the PCR primer × 5 (for food × 1 / for ACTN 3 × 1 / remaining 3 determined by the investor), the DNA extraction reagent × 50, the real-time PCR reagent × 50, PCR test introductory book · laboratory pipette · 2 pipette tip protection boxes · PCR tube · sterile cotton swab for DNA collection set as "The Biohacker Kit"Is also available.


The following are examples of diagnosis and analysis possible by "Open qPCR".
·Genotyping:Examination of exercise capacity,BRCA geneGenotype of genotypes required for breed improvement of animals based on desired genotype
· Food safety:Survey of Salmonella and Escherichia coli in food, water quality survey, whether horse meat is used for meat of hamburger, dolphin meat is not used in tuna can
·Investigation:Analysis of cellular gene expression level, design of biological system by DNA manipulation / rearrangement
·health:Virus surveillance of HIV · Ebola, malaria ·West Nile virusTesting the insect vector such as


If you invest 1,499 dollars (about 172,000 yen), you can get one qPCR for 100 people only, with $ 1699 (approx. 195,000 yen) and 200 qPCR for 2nd shipment only for 200 people It is possible to get. You can also add one set of The Biohacker Kit for $ 249 (about 28,500 yen). We can not get any other equipment, but every time we get 10 dollars of capital of $ 140 (about 16,000 yen), we will donate one qPCR to a clinic in developing countries such as West Africa and an organization that tackles Ebola outbreak heat treatment "DONATE TO A plan such as FIGHT EBOLA (Ebola support donation) is also available.


Product shipping will begin around May 2015, shipping fee is free anywhere in the world. The deadline of investment is 11:00 am on Friday, December 5, 2014.

Open qPCR: DNA Diagnostics for Everyone by Chai Biotechnologies - Kickstarter
https://www.kickstarter.com/projects/chaibio/open-qpcr-dna-diagnostics-for-everyone