Succeeded in developing world's first and tuberculosis-resistant cattle using genetic editing technology "CRISPR"

ByKabsik Park

Research using gene editing technology "CRISPR / Cas9", the world's first "Bovine tuberculosisIt succeeded in producing a cow that succeeded in raising the resistance of the cow. "

Tuberculosis-resistant cDNA development for the first time using CRISPR technology | Science Bulletin
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A research group conducting veterinary research at the Northwest Agricultural Science and Technology University in China succeeded in developing cattle resistant to bovine tuberculosis using "CRISPR / Cas 9" is a biological related academic journalGenome BiologyAboveAnnouncementDid.

"CRISPR / Cas9" is a genome editing technique applying repetitive clusters contained in DNA, and it is clear how to read about the following article.

What is the genetic editing technology "CRISPR" and what is the future of humanity and humanity? - GIGAZINE


Dr. Young Chan, the first author of the paper, said, "By using the CRISPR system called the latest CRISPR / Cas 9n, we can insert a tuberculosis resistance gene called" NRAMP 1 "into the genes of cows Succeeded. The important thing in this experiment is that it is important for our cow geneOff target effectIt is the point that CRISPR technology we used could be adapted to the production of genetically modified livestock, "he says.

CRISPR technology has been widely used in research institutions and the like in recent years, and it is known for being able to rewrite genes very accurately and easily. However, sometimes it is unintentionally thought that off-target effects are developed, so there was a need in the field of genetic research for methods that do not express this or that reduce the expression rate.

ByMaciej Lewandowski

In his own research, Dr. Chan said, "When trying to insert a new gene into the genome of a mammal," finding a suitable place for genetic insertion in the genome "is very difficult. It is necessary to look for "in places where minimal effects occur even if other genes enter" in the genome.We are suitable for inserting genes by using a close and methodological approach I have identified the place, and it has not been confirmed that the off-target effect has been confirmed after inserting the gene, "succeeded in suppressing the expression of the off-target effect, which his own research was difficult before. We are saying that.

In the experiment, "NRAMP 1" gene was inserted into female cow's cow using "CRISPR / Cas 9 n" technique. After that, it seems that the cell into which the gene was inserted was used as "donor cell" for somatic cell nuclear transplantation. A calf with this "CRISPR gene inserted gene" was produced, 11 of which were resistant to tuberculosis and did not have a genetic disorder, that is, off-target effect did not develop It was done. Furthermore, genetic analysis of the 11 babies born has confirmed that "NRAMP 1" is also tightly integrated into the target part of the genetic code.

ByThomas Wensing

In addition, exposure of eleven calves to bacterial "M. bovis" which is a source of infection of bovine tuberculosis showed that the resistance of the calves in the body increased from the survey using blood samples Has also been confirmed. About this, Dr. Chan said, "Our research was the first to demonstrate that we can produce genetically modified animals without off-target effects," our study was the first study, Is also related to finding where genetic editing is applied to the gene in the bovine genome and the inserted gene is useful ".